Yes I believe they did, Tsunade healed Sasuke after Itachi put him through the tsukuyomi but I am not sure they talked and Tsuande was in a coma during the kage summit so she wasn't there.
Omg how could I forget. XD That was a short meeting though. The only thing she was able to see was Sakura's affection for Sasuke, which is quite different now. I wonder if she will take notice of that, kind of like Sai did.